PRODUCT# 105-40:  Recombinant M. tuberculosis MTP-64 Antigen (E.coli)

SPECIFICATIONS                                                             APPLICATIONS

  Concentration: see vial                                                     ELISA

  Mass/vial: see vial                                                             Western ELISA 

  Diluent: 50mMTris, 0.1M Nacl, pH 8.5                               TB Diagnostics 

 Purity: >98%                                                                        Immunization

  Stabilizer: None     

  Preservative: None  

  Storage: -75C

  Physical State: Frozen Liquid

  Stability: 24 months at 75oC.

DESCRIPTION: M. tuberculosis (virulent strain) MTP-64 antigen – synthetic gene -produced in the E. coli expression system.    

PURIFICATION: Purified by His Select affinity chromatography to >98%  purity as determined by SDS-PAGE, reduced. MW: approx. 33.9kD    

SPECIFICITY:     This MTP-64 protein reacts with M. tuberculosis converted human serum in ELISA and Western ELISA. Suitable for diagnostic applications.

BIOLOGICAL ACTIVITY:  Not done

APPLICATION AND INSTRUCTIONS FOR USE

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.