Product Specifications:

Item # 1121-F: Fluorescene Murine Anti-gp120 HIV-1 mAb IgG (Cross Reactive)

Mass/vial: Approximately 50µg IgG2b

Vol/vial: 100ul

Diluent: 10mM Tris, pH 8.0                                  

Stabilizer: None                                                       

Preservative: None                                                       

Storage: -75°C                                                                             

F/P Ratio: 3 - 4

Physical State: Frozen Liquid

Stability: At least 12 months at -75°C

Minimum Dilution: 1 : 50

Applications: Immunohistocytology, Immunostaining/sorting, Dot Blots, FACS.

Description: Fluorescene conjugated Murine Anti-gp120, V3 loop specific, HIV-1 cross reactive mAb.

Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.

Specificity: This fluorescene conjugated mAb binds to native and recombinant HIV-1 gp120 (IIIB, MN, SF2, RF, CDC4) in Dot Blot assays and stains cell surface bound gp120 in HIV-1 infected SupT1 and primary HIV-1 infected human PBLs.

Biological Activity: None

Application and Instruction for use:

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range.  Dot Blot assays with purified gp120 may be performed in 1 : 200 dilution range.