Product Specifications:

Item#1007-G: vif HIV-1 IIIB Conjugated to Colloidal Gold

Concentration: See vial 

Volume/vial: 1ml Gold Conjugate

Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3 

Storage: 4°C

Stability: At least 3 months at 4°C Minimum

Dilution: 1 : 50

Application: Dot Blot Assay-Anti-vif Antibody Binding, Immunohistochemistry. 

Description: vif HIV-1 IIIB conjugated to Colloidal Gold (25nm mean particle size).

Coupling Ratio: OD 520nm = 10; protein content approximately 25µg.

Specificity: Gold conjugated vif binds to murine monoclonal antibodies of defined epitope specificity and human/rabbit polyclonal antibodies as determined by dot blot assays.

Biological Activity: Not determined.

Application and Instructions for use:

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. Dot blot assays may be performed in 1 : 100 dilution range depending on the concentration of solid phase CD4/Anti-gp120 in the test system.

2. Immunohistochemical studies may be performed in 1 : 50 dilution range.