PRODUCT SPECIFICATIONS

PRODUCT#1102-G: Murine Anti-tat HIV-1 mAb Conjugated to Colloidal Gold

SPECIFICATIONS                                                 APPLICATIONS

  Mass: Approximately 50µg  Protein                 Dot Blot Assays - tat Binding

  Volume/vial: 1ml Gold Conjugate                      Immunohistochemistry

  Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG;

0.02% NaN3  

  Storage: 4oC   

  Stability: At least 3 months at 4oC

  Minimum Dilution: 1 : 100

DESCRIPTION: Murine Anti-tat HIV-1 mAb conjugated to Colloidal Gold (25nm mean particle size).

COUPLING RATIO: OD 520nm = 15; protein content approximately 50µg..

SPECIFICITY: Gold conjugated Anti-tat binds to tat (HIV-1) in dot blot assays and HIV-1 infected SupT1 cells - permeabelized.

BIOLOGICAL ACTIVITY: Not determined .

APPLICATIONS AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

Gold conjugated anti-tat may be used in 1 : 100 dilution range depending on the concentration and speicificity of the test ligand (tat).  Immunohistochemstry may be performed in 1 : 50 dilution range.

RELATED PRODUCTS

PRODUCT#1102: Murine Anti-tat mAb

Application:  tat ELISA, tat capture ELISA, Drug Screening, Diagnostics.

PRODUCT#1102-G: Colloidal gold-Conjugated Murine Anti-tat mAb

Application:  Drug screening, Histochemistry, tat Dot Blot.

PRODUCT# 1102-F: Flourescene-Conjugated Murine Anti-tat mAb