Product Specifications:
Item#1106-G: Murine anti-p66 (RT) HIV-1 Monoclonal Antibody Conjugated to Colloidal Gold
Antibody: IgG1
Concentration: See vial
Mass/vial: N/A
Diluent: 10mM Tris, pH 8; 5% BSA; 0.05 PEG; 0.02% NaN3
Storage: 4°C
Stability: At least 3 months at 4°C Minimum
Dilution: 1 : 50
Application: Dot Blot Assays, Immunohistocytology.
Description: High affinity murine anti-p66 HIV-1 mAb conjugated to Colloidal Gold (approx. 45nm mean diameter).
Coupling Ratio: OD 520nm = 15; protein content approximately 50µg.
Specificity: Binds to native and recombinant p66 (RT) HIV-1 as determined by dot blot assays.
Biological Activity: Not determined.
Application and Instructions for use:
Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Dot Blot assays may be performed at 10-100ng of p66 in solid phase (membrane bound). Immunohistochemical studies may be performed in 1:50 to 1:100 dilution range of the gold conjugate.