PRODUCT SPECIFICATION

Product# 5103-F:  Fluorescene Murine anti-p27 SIVmac251 Monoclonal Antibody

SPECIFICATIONS                                                              APPLICATIONS

  Antibody Class: IgG1                                                      FACS

  Mass/vial: Approx. 50ug (50ul)                                    Immunohistocytology

  Diluent: 10mM Tris, pH8.0,10mM NaCl

  Stabilizer: None

  Preservative: None

  Physical State: Frozen Liquid

  F/P Ratio: 3-4

  Storage: -75oC

  Stability: At least 12 months

at -75oC

  Minimum Dilution: 1:50

DESCRIPTION: Fluorescene conjugated murine anti-p27 SIV mAb.

PURIFICATION: Highly purified mAb was used for FITC conjugation and the conjugate purified

                                           by gel exclusion chromatography..

SPECIFICITY: Binds to recombinant SIV p27 and with SIV lysates in immunoblots and stains     

                                           SIV infected T-cells.

APPLICATIONS AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range.  Dot Blot assays with purified p27 may be performed in 1 : 200 dilution range.

RELATED PRODUCTS

PRODUCT#:  5103-P Peroxidase-Conjugated Murine anti-p27 SIV mAb

Application:  ELISA, Western ELISA, Histocytochemistry, RIPA.

PRODUCT#:  5103-B Biotin  Murine anti-p27 SIVmAb

Application:  FACS, Cell Staining of SIV infected cells, Elisa, Western.