Product Specifications:

Item # 1001-F:  Fluorescene rgp120 HIV-1 IIIB

Mass/vial: Approximately 50µg gp120                                  

Diluent: 10mM Tris, pH 8.0                                  

Stabilizer: None                                                       

Preservative: None                                                       

Storage: -75°C                                                      

Physical State: Frozen Liquid                                           

F/P Ratio: 4 -5

Stability: 6 months at -75°C

Minimum Dilution: 1 : 50

Applications: Direct Immunofluorescence, Immunohistocytology, FACS, Dot Blots

Description: Baculovirus derived rgp120 HIV-1 IIIB conjugated to FITC.

Purification: Fluorescene conjugated rgp120 was purified by gel exclusion chromatography and UF concentrated.

Specificity: This fluorescene conjugated gp120 binds to murine monoclonal antibodies of defined epitope specificity.

Biological Activity: This fluorescene conjugated gp120 binds to CD4 bearing T-cells  as determined by  FACS.

Application and Instruction for use:

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining may be performed in 1 : 50 dilution range.  Dot Blot assays with purified anti-gp120 monoclonal/ rabbit polyclonal antibodies may be performed at a minimum dilution of 1 : 100.