Product Specifications:

Item # 1002-F:  Fluorescene tat HIV-1

Mass/vial: At least 50µg tat                        

Diluent: 50mM Tris, 20mM Nacl, PH8                                  

Stabilizer: None                                                       

Preservative: None                                                       

Storage: -75°C                                                      

Physical State: Frozen Liquid                                           

F/P Ratio: 4 -5

Stability: 6 months at -75°C

Minimum Dilution: 1 : 100

Applications: Direct Immunofluorescence, Immunohistocytology, FACS, Dot Blots

Description: E.coli derived tat (HIV-1 ) conjugated  with FITC.

Purification: Fluorescene conjugated tat was purified by gel exclusion chromatography and UF concentrated.

Specificity: This fluorescene conjugated tat binds to murine monoclonal antibodies of defined epitope specificity.

Biological Activity: Binding to human Hexim has not been determined.

Application and Instruction for use:

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining may be performed in 1 : 50 dilution range.  Dot Blot assays with purified anti-gp120 monoclonal/ rabbit polyclonal antibodies may be performed at a minimum dilution of 1 : 100.