Product Specifications:

Item # 1004-F: Fluorescene rgp160 HIV-1 IIIB

Mass/vial: At least 50µg rgp160      

Diluent: 10mM Tris, pH 8.0                                  

Stabilizer: None                                                       

Preservative: None                                                       

Storage: -75°C                                                                             

F/P Ratio: 2 - 4

Stability: At least 6 months at -75°C

Minimum Dilution:   1 : 50

Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence

Description: Baculovirus derived rgp160 (HIV-1 IIIB) conjugated to FITC.

Purification: Fluorescene conjugated rgp160 was purified by gel exclusion chromatography and UF concentrated.

Specificity: This fluorescene conjugated rgp160 binds to murine monoclonal antibodies of defined epitope specificity and immobilized CD4 in Dot Blot assays.

Biological Activity: This fluorescene conjugated gp160 binds to CD4 bearing cells in FACS.

Application and Instruction for use:

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of CD4 bearing cells may be performed in 1 : 50 dilution range.  Dot Blot assays with purified anti-gp160 monoclonal/polyclonal antibodies may be performed at a minimum dilution of 1 : 100.