Product Specifications:

Item # 11011-F: Fluorescene Murine Anti-gp120 HIV-1 IIIB mAb IgG

Mass/vial: Approximately 50µg IgG

Vol/vial: 50ul

Diluent: 10mM Tris, pH 8.0                                  

Stabilizer: None                                                       

Preservative: None                                                       

Storage: -75°C                                                                             

F/P Ratio: 3 - 4 

Stability: At least 6 months at -75°C

Minimum Dilution:   1 : 50

Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence

Description: Fluorescene conjugated Murine Anti-gp120, V3 loop specific (HIV-1 IIIB) mAb.

Purification: Highly purified mAb IgG (>95% pure) was used for FITC conjugation and the conjugate purified by gel exclusion chromatography.

Specificity: This fluorescene conjugated mAb binds to native and recombinant HIV-1 gp120 in Dot Blot assays and stains cell surfaces in direct immunofluorescence assays.

Biological Activity: None

Application and Instruction for use:

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified gp120 may be performed in 1 : 200 dilution range.