Product Specifications:

Item # 1007-F: Fluorescene vif HIV-1

Mass/vial: At least 50µg vif  

Diluent: 10mM Tris, pH 8.0                                  

Stabilizer: None                                                       

Preservative: None                                                       

Storage: -75°C                                                                             

F/P Ratio: 2 - 4

Stability: At least 6 months at -75°C

Minimum Dilution:   1 : 50

Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence

Description: E.coli derived vif (HIV-1 ) conjugated to FITC.

Purification: Fluorescene conjugated vif was purified by gel exclusion chromatography and UF concentrated.

Specificity: This fluorescene conjugated vif binds to murine monoclonal antibodies of defined epitope specificity.

Biological Activity: ND

Application and Instruction for use:

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of CD4 bearing cells may be performed in 1 : 50 dilution range.  Dot Blot assays with purified anti-gp160 monoclonal/polyclonal antibodies may be performed at a minimum dilution of 1 : 100.