Product Specifications:

Item# 1001-B: Biotin rgp120 HIV-1 IIIB

 Mass/Vial: 50µg                                                                    

 Vol./Vial: 50ul                                                                    

 Diluent: PBS+ 0.2% BSA, pH 7.5                                    

 Stabilizer: BSA                                                               

 Preservative: 0.1% NaN3                                                              

 Storage: 4°C                                                                          

 Stability: 6 Months

 Minimum Dilution:1 : 2,000

 Application: T-Cell Research, Diagnostics, ELISA, Western ELISA, Drug Screening.

Description: Biotin conjugated to rgp120 HIV-1 IIIB.

 Coupling Ratio: Approximately 5-20 moles of biotin per mole gp120. 

 Specificity: Biotin gp120 binds to Murine and Human Anti-gp120 mAbs as determined by strepavidin-HRP  ELISA and Western ELISA assays.

 Biological Activity: Biotin gp120 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.

 Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. ELISA may be performed in 1 : 10,000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents.  gp120/CD4 capture ELISA may be performed in 100ng CD4 in solid phase and 0.1-10ng of biotin-gp120.