Product Specifications:

Item# 1021-B: Biotin rgp120 HIV-1 MN 

 Mass/Vial: 50µg

 Vol./Vial: 50µl 

 Diluent: PBS + 0.2% BSA 

 Stabilizer: BSA

 Preservative: 0.1%NaN3 

 Storage: 4°C

 Stability: Not Determined

 Minimum Dilution: 1 : 2000

 Applications: T-Cell Research, ELISA, Western ELISA, Diagnostics, Drug Screening.

Description: Biotin conjugated to rgp120 HIV-1 MN.

 Coupling Ratio: Approximately 10-20 moles of biotin per mole gp120.

 Specificity: Biotin gp120 MN binds to Murine anti-gp120 mAbs (structural and non-structural epitopes) as determined by biotin- strepavidin-AP/HRP ELISA and Western ELISA assays.

 Biological Activity: Biotin gp120 binds to solid phase CD4 as determined by capture ELISA using strepavidin-HRP as a secondary reagent.

 Application and Instructions for use:

 Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. ELISA may be performed in 1 : 10,000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. gp120/CD4 capture ELISA may be performed in 100ng CD4 in solid phase and .1-1.0ng of biotin-gp120.