Product Specifications:

Item# 1103-B: Biotin Murine Anti-p24 HIV-1 IIIB mAb IgG

Mass/vial: Approximately 50µg IgG 

Volume: 50µl

Diluent: PBS

Stabilizer: 0.1%BSA 

Preservative: 0.02% Azide

Storage: 4°C

Physical State: Liquid

B/P Ratio: 10 - 20

Stability: At least 6 months at 4°C

Minimum Dilution: 1 : 2000

Application: Dot Blots, FACS, Immunohistocytology, Direct Immunofluorescence.

Description: Biotin conjugated Murine anti-p24 (HIV-1 IIIB) mAb.

Purification: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.

Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 p24 in Dot Blot assays, ELISA and Western ELISA.

Biological Activity: None

Application and Instructions for use:

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg p24 range with 1-2µg/ml conjugate.

Related Products:

Item# 1103: Murine mAb p24 HIV-1 IIIB

Application: ELISA, Western ELISA, Immunoprecipitation, Immunohistochemistry.

Item# 1103-P: Peroxidase-Conjugated Murine mAb p24 HIV-1 IIIB

Application: Diagnostics, ELISA, Western ELISA.

Item# 1103-G: Colloidal Gold-Conjugated Murine mAb p24 HIV-1 IIIB

Application: Diagnostics, Dot Blot, Histocytology.