Product Specifications: Item # 1106-B: Biotin Murine Anti-p66 (RT) HIV-1 mAb IgG Mass/vial: Approx 50µg IgG Volume: 50µl Diluent: PBS Stabilizer: 1% BSA Preservative: 0.1% Azide Storage: 4°C Physical State: Liquid B/P Ratio: 10 - 20 Stability: At least 6 months at 4°C Minimum Dilution: 1 : 5000 Applications: ELISA/ Western ELISA, FACS, Immunohistocytology, Description: Biotin conjugated Murine anti-p66 (RT) HIV-1 mAb. Purificatiion: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography. Specificity: This biotin conjugated mAb binds to native and recombinant HIV-1 p66 in Dot Blot assays, ELISA and Western ELISA. Biological Activity: None Application and Instructins for use Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. Western ELISA may be performed in 10-100pg p66 range with 1:500 dilution of the conjugate. |
