PRODUCT SPECIFICATIONS

PRODUCT # 1108-B:  Biotin Murine Anti-Nef HIV-1 mAb IgG

SPECIFICATIONS                                         APPLICATIONS

Mass/vial: Approximately 50µg                    IgG Elisa/Westerns

Volume: 50µl                                                  FACS

Diluent: PBS                                                 Immunohistocytology

Stabilizer: 1% BSA

Preservative: 0.1% Azide

Storage: 4oC

Physical State: Liquid  

B/P Ratio: 5-10

Stability: At least 6 months at 4oC.

Minimum Dilution: 1 : 2000                                    

DESCRIPTION: Biotin conjugated Murine anti-Nef HIV-1 mAb.

PURIFICATION: Highly purified mAb IgG (>95% pure) was used for Biotin conjugation and the conjugate purified by gel exclusion chromatography.

SPECIFICITY: This biotin conjugated mAb binds to native and recombinant HIV-1 Nef in ELISA and Western ELISA.

BIOLOGICAL ACTIVITY: None

APPLICATION AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA may be performed in 1 : 2000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents.  Western ELISA may be performed in 10-100pg Nef range with 1:500 dilution of the conjugate.