Product Specifications:

Item#1302-G: Rabbit Anti-tat HIV-1 pAb Conjugated to Colloidal Gold

 Mass/vial: Approximately 50µg Protein

 Volume/vial: 1ml Gold Conjugate

 Diluent: 10mM Tris, pH8;5% BSA;0.05% PEG; 0.02% NaN3

 Storage: 4°C

 Stability: At least 6 months at 4°C Minimum

 Dilution: 1 : 100

 Application: Dot Blot Assays - tat Binding, Immunohistochemistry.

 Description: Rabbit anti-tat HIV-1 pAb conjugated to Colloidal Gold (25nm mean particle size).

 Coupling Ratio: OD 520nm = 15; protein content approximately 50µg.

 Specificity: Gold conjugated anti-tat binds to native and recombinant tat (HIV-1, IIIB) in dot blot assays.

 Biological Activity: Not determined.

 Application and Instructions for use:

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Gold conjugated anti-gp120 may be used in 1 : 100 dilution range depending on the concentration and speicificity of the test ligand (gp120). Immunohistochemstry may be performed in 1 : 50 dilution range.

 Related Products:

 Item#: 1302-P Peroxidase-Conjugated Rabbit anti-tat HIV-1 IIIB

 Application: ELISA, Western ELISA, Histocytochemistry, Histocytology.

 Item#: 1302-F Fluorescene Rabbit anti-tat HIV-1 IIIB

 Application: FACS, Cell Stainin

 Item#: 1302-M Magnetic Rabbit anti-tat HIV-1 IIIB

 Application: Immunoprecipitation, Cell Panning.