Product Specifications:

Item#1303-G: Rabbit Anti-p24 HIV-1 pAb Conjugated to Colloidal Gold

Mass/vial: Approximately 50µg Protein

 Volume/vial: 1ml Gold Conjugate

 Diluent: 10mM Tris, pH8;5% BSA;0.05% PEG; 0.02% NaN3

 Storage: 4°C

 Stability: At least 3 months at 4°C Minimum

 Dilution: 1 : 100

 Application: Dot Blot Assays - p24 Binding, Immunohistochemistry.

 Description: Rabbit anti-p24 HIV-1 pAb conjugated to Colloidal Gold (25nm mean particle size).

 Coupling Ratio: OD 520nm = 15; protein content approximately 50µg.

 Specificity: Gold conjugated rabbit anti-p24 binds to native and recombinant p24 (HIV-1, IIIB) in dot blot assays.

 Biological Activity: Not determined.

 Application and Instructions for use:

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Gold conjugated anti-p24 may be used in 1 : 100 dilution range depending on the concentration and speicificity of the test ligand (p24). Immunohistochemstry may be performed in 1 : 50 dilution range.

 Related Products:

 Item#: 1303-P Peroxidase-Conjugated Rabbit anti-p24 HIV-1 IIIB

 Application: ELISA, Western ELISA, Histocytochemistry, Histocytology.

 Item#: 1303-F Fluorescene Rabbit anti-p24 HIV-1 IIIB

 Application: FACS, Cell Staining

 Item#: 1303-M Magnetic Rabbit anti-p24 HIV-1 IIIB

 Application: Immunoprecipip24ion, Cell Panning.