Product Specifications: Item# 1041-P: Peroxidase-Conjugated rgp120 HIV-1 IIIB (CHO) Concentration: See vial Mass/vial: 75µg Volume/vial: 100µl Diluent: 50mM Tris, pH 8 1% BSA Preservative: 0.01% Thimerasol Storage: 4°C Stability: At least 3 months at 4°C Minimum Dilution: 1 : 1000 Application: ELISA/Western ELISA, Human T-cell Research, Histocytochemistry, Drug Screening. Description: Peroxidase conjugated full length rgp120 HIV-1 IIIB (CHO). Molar Ratio: 2 - 4 peroxidase molecules per molecule of gp120. Specificity: Peroxidase conjugated rgp120 IIIB (CHO) binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western ELISA Biological Activity: Peroxidase conjugated rgp120 IIIB (CHO) binds to immobilized human T-cell receptor CD4 in ELISA and Western ELISA. Application and Instructions for use Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. 1. ELISA and Western ELISA may be performed at dilutions of 1 : 1000 depending on the concentration of CD4 in the test system. Binding to murine and human polyclonal antibodies may be performed at higher dilutions. |