Product Specifications:

Item# 1002-B: Biotin rtat HIV-1 IIIB 

 Mass/Vial: 50µg 

 Vol./Vial: 100µl 

 Diluent: PBS 

 Stabilizer: 1% BSA 

Preservative: Thimersol 

 Storage: 4°C

 Stability: 6 Months  

 Minimum Dilution: 1 : 1000

 Application: T-Cell Research, Diagnostics, Drug Screening, ELISA, Western ELISA.

 Description: Biotin conjugated to tat HIV-1 (E.coli). COUPLING RATIO: Approximately 10-20 moles of biotin per mole tat.

 Biological Activity: Biotin tat binds to solid phase murine anti-tat mAb as determined by capture ELISA using strepavidin-HRP as a secondary reagent.

 Application and Instructions for use:

 Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. ELISA may be performed in 1 : 1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents. tat/anti-tat mAb capture ELISA may be performed in 100ng mAb in solid phase and 1-10ng of biotin-tat.