PRODUCT SPECIFICATIONS

Product# 1081-B:  Biotin rgp120 HIV-1 ADA

 SPECIFICATIONS                                                    APPLICATIONS

  Mass/Vial: 50µg                                                     T-Cell Research

  Vol./Vial: 50µl                                                         Diagnostics

  Diluent: PBS                                                          Drug Screening

  Stabilizer: 0.2% BSA                                            ELISA, Western ELISA

  Preservative: 0.1%NaN3     

  Storage: 4oC   

  Stability: Not Determined

  Minimum Dilution: 1 : 2000

DESCRIPTION:     Biotin conjugated to rgp120 ADA

COUPLING RATIO:   Approximately 10-20 moles of biotin per mole gp120.  

SPECIFICITY:       Biotin gp120 ADA binds strepavidin-AP/HRP in ELISA and                                      Western ELISA.

BIOLOGICAL ACTIVITY:      Biotin gp120 binds to solid phase CD4 as determined by capture ELISA using   strepavidin-HRP as a secondary reagent.

APPLICATION AND INSTRUCTIONS FOR USE

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

1. ELISA may be performed in 1 : 1000 dilution range depending on the concentration of the test ligand and specificity and sensitivity of the secondary reagents.  gp120/CD4 capture ELISA may be performed in 100ng CD4 in solid phase and .1-1.0ng of biotin-gp120.