PRODUCT SPECIFICATION

Product# 7108:  Murine Anti-Rhesus TRIM5 Alpha Monoclonal Antibody

  SPECIFICATIONS                                                  APPLICATIONS

  Antibody Class: Clone 9, IgG                              ELISA, Western ELISA

  Concentration: See vial                                       Immunofluoresence

  Mass/vial: 100µg                                                 Immunohistochemistry

  Purity: >95%  

  Stabilizer: None  

  Preservative: None  

  Physical State: Frozen Liquid

  Storage: -75oC

  Stability: At least 24 months at -75oC.

DESCRIPTION: High affinity murine Anti-RhTRIM5 alpha  mAb purified by protein A/G affinity chromatography to >95% purity, as determined by DSD-PAGE, reduced.

SPECIFICITY: Binds to native and recombinant Rh Trim5alpha as  determined by ELISA, Western ELISA and Immunofluorescense studies. 

BIOLOGICAL ACTIVITY: None.

APPLICATIONS AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays.  Western ELISA require at 1-5µg of mAb/ml. Immunofluorescense studies were performed at 2-5ug/10E6 cells.