Product Specifications:

Item# 1103T: Murine anti-p24 HIV-1 Monoclonal Antibody 

Antibody Class: IgG

Concentration: See vial 

Mass/vial: 100µg

Purity: >95% 

Stabilizer: None

Preservative: None

Physical State: Liquid

Storage: -75°C

Stability: At least 12 months at -75°C

Application: ELISA,Western,ELISA, Immunoprecipitation,Immunocytochemistry, Immunohistochemistry.

Description: High affinity murine anti-p24 HIV-1 IIIB mAb.

Specificity: Reacts with native and recombinant HIV-1 p24 in Elisa and Western Elisa. Detects HIV-1 p24 in capture Elisa assays.

Biological Activity: Not determined.

Application and Instructions for use 

Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA may be performed at 10-100ng of mAb depending on the amount of immobilized antigen in the assays. Western ELISA requires 1-5µg of mAb. 

Item#: 1103-P Peroxidase-Conjugated Murine anti-p24 HIV-1 IIIB

Application: ELISA, Western ELISA, Histocytochemistry, Histocytology.

Item#: 1103-F Fluorescene Murine anti-p24 HIV-1 IIIB

Application: Cell Staining

Item#: 1103-G Colloidal Gold-Conjugated Murine anti-p24 HIV-1 IIIB

Application: Dot Blots, EM.

Item#: 1103-M Magnetic Murine anti-p24 HIV-1 IIIB

Application: Immunoprecipitation.

Research paper related to Murine mAb p24 HIV-1 Clone 4F6

A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24

MicroPlex carboxymethylated microspheres region 42 (5.6μm in diameter, catalog number L100-C142-01, Luminex Corporation, Austin, TX) were coupled to a high affinity anti-p24 monoclonal antibody (catalog # 1103-clone 4F6, ImmunoDiagnostics, Inc., Woburn, MA).