PRODUCT SPECIFICATION

Product# 1102:  Murine Anti-tat HIV-1 IIIB Monoclonal Antibody

SPECIFICATIONS                                                       APPLICATIONS

  Antibody Class: IgG1                                               ELISA, Western ELISA

  Concentration: See vial                                            Immunoprecipitation

  Mass/vial: 100µg                                                       Immunocytochemistry

  Purity: >95%                                                             Virus Neutralization in culture

  Stabilizer: None  

  Preservative: None  

  Physical State: Frozen Liquid

  Storage: -75oC

  Stability: At least 12 months  at -75oC

 DESCRIPTION: High affinity murine anti-tat HIV-1.

SPECIFICITY: Binds to native and recombinant HIV-1 tat in ELISA and Western ELISA. Epitope maaped to N-terminus of HIV-1 tat.

BIOLOGICAL

ACTIVITY: Neutralizes tat dependent HIV-1 replication in infected monocytic cells.

APPLICATIONS AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays.  Western ELISA requires 1-5µg of mAb/ml.

RELATED PRODUCTS

PRODUCT#:  1102-P Peroxidase-Conjugated Murine anti-tat HIV-1 IIIB

Application:  ELISA, Western ELISA, Histocytochemistry.

PRODUCT#:  1102-F Fluorescene Murine anti-tat HIV-1 IIIB

Application:  FACS, Cell Staining

PRODUCT#:  1102-G Colloidal Gold-Conjugated Murine anti-tat HIV-1 IIIB

Application:  Dot Blots, EM.

PRODUCT#:  1102-M Magnetic Murine anti-tat HIV-1 IIIB

Application:  Immunoprecipitation.