PRODUCT SPECIFICATION

Product# 1101:  Murine anti-gp120 IIIB Monoclonal Antibody

SPECIFICATIONS                                                   APPLICATIONS

Antibody Class: IgG1                                             ELISA, Western ELISA

Concentration: See vial                                          Immunoprecipitation

Mass/vial: 100µg                                                    Immunocytochemistry

Purity: >95%                                                          Immunohistochemistry

Stabilizer: None                                                      In Vitro Virus Neutralization

Preservative: None  

Physical State: Frozen Liquid

Storage: -75oC

Stability: At least 12 months at -75oC

DESCRIPTION: High affinity murine anti-gp120 IIIB mAb.

SPECIFICITY: Binds to V3-loop of HIV-1 IIIB gp120/gp160.  This mAb shows 5-10% cross reactivity with other HIV-1 strains.

BIOLOGICAL

ACTIVITY: Neutralizes HIV-1 IIIB at 1-10µg/ml as determined by syncycium assays with CD4-bearing HIV-1 IIIB infected cells and co-cultures of infected PBLs.

APPLICATIONS AND INSTRUCTIONS FOR USE

Recommended dilutions for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA may be performed at 10-100ng/ml of mAb depending on the amount of immobilized antigen in the assays.  Western ELISA requires 1-5µg of mAb/ml.

RELATED PRODUCTS

PRODUCT#:  1101-P Peroxidase-Conjugated Murine anti-gp120 HIV-1 IIIB

Application:  ELISA, Western ELISA, Histocytochemistry, Histocytology.

PRODUCT#:  1101-F Fluorescene Murine anti-gp120 HIV-1 IIIB

Application:  FACS, Cell Staining

PRODUCT#:  1101-G Colloidal Gold-Conjugated Murine anti-gp120 HIV-1 IIIB