Product Specifications:

Item# 30090: Recombinant Dengue Antigen D4 Envelope Protein (E.coli)

Concentration: see vial

Mass/vial: see vial 

Solids: 50mM NaCl,50mM Tris, pH8.5, 0.02%SDS

Purity: >95%

Stabilizer:  None

Preservative: None

Storage: -75⁰C

Physical State: Frozen  Liquid

Stability: At least 36 months at -75⁰C

Applications: Elisa/Western Blot, Dengue Diagnostics

Description: Synthetic Dengue virus D2 Envelope DNA sequence, amino acid residues 1-416, expressed as a fusion protein in the E.coli Expression System.

Purification: This protein is purified by Ni affinity chromatography to >95% purity as determined by SDS-PAGE, reduced.

Molecular Weight: approx. 75kD.

Specificity: This protein binds to murine monoclonal antibodies – structural epitopes, and to Dengue virus converted human serum antibodies as determined by Elisa, Western Blots and by one-step lateral flow diagnostic testing.

Application and Instructions for use:

Recommended concentrations for use are approximate values. Elisa is performed in 1-2ug/ml protein range for tittering Dengue virus converted human polyclonal antibodies. Concentrations if 1-2mg/ml (1-2ul/cm) are recommended for Lateral Flow Diagnostics.