Product Specifications:

Item# 15009:  Recombinant Treponema pallidum TmpA Membrane Protein (E.coli)

Concentration: See vial                                                             

Mass/vial: See vial                                                   

Volume/vial: See vial                                                          

Diluent: 10 mM Tris, pH 8.0, SDS (<0.01%)                                                                

Purity: >95%                                                                                                    

Stabilizer: None                                                                        

Preservative: None                                                                     

Storage:  -75°C

Physical State:Frozen Liquid

Stability:  2 years at -75°C 

Application: In-Vitro Diagnostics, Immunization

Description: Recombinant Treponema pallidum TmpA membrane protein produced as a fusion  protein in the E.coli Expression System. Fusion protein

molecular Weight: 68kD.

Purification: This protein is purified by affinity chromatography and by preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.

Specificity: This protein binds to murine anti-TmpA protein monoclonal antibodies and Treponema pallidum converted human serum polyclonal antibodies in ELISA and Western Elisa, Dot Blots and Lateral Flow immunochromatographic diagnostic tests.

Biological Activity:  Not done

Applications and Instructions for use

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml.