Product Specifications:

Item# 20002: Recombinant T. cruzi (Chagas) Microtubule-Associated Protein, MAP

Concentration:  See Vial                                                  

Mass/vial: See vial                                                

Volume/vial: See Vial                                                          

Diluent: PBS, 5% glycerol                                                                  

Purity: >95%                                                                                                    

Stabilizer: None                                                                        

Preservative:None                                                                     

Storage: -75°C

Physical State: Frozen Liquid

Stability: 2 years at -75°C 

Application: In-Vitro Diagnostics, Immunization.

Description: Recombinant T. cruzi (Chagas) Microtubule-Associated Protein, MAP produced in the E.coli Expression System.

Purification:This protein is purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.

 Approx. Molecular Weight: 20.1kD.

Specificity: This protein binds to T. cruzi (Chagas) converted human serum polyclonal antibodies in ELISA and Western Elisa. Qualified for one-step immunochromatographic diagnostic tests.

Biological Activity: Not done

Application and Instructions for use

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml, 1ul/cm.