Product Specifications:

Item# 20004: Recombinant T. cruzi (Chagas) Composite Antigen A13, B13,H49,1F8 Antigen (E.coli)

Concentration:  NA                                               

Mass/vial: 1mg/ml, ml                                              

Volume/vial: 1ml                                                    

Diluent: 50mM Tris, 50mM NaCl, pH 8.3, SDS trace                                                 

Purity: >98%                                                                                                    

Stabilizer: None                                                                        

Preservative:None                                                                     

Storage: Short term -20°C, long term -75°C

Physical State: Frozen Liquid

Stability: 2 years at -75°C

Application: In-Vitro Diagnostics, Immunization.

Description: Recombinant T.cruzi (Chagas) combination A13, B13, H49 and 1F8 antigen expressed in the E.coli Expression System.

Purification:This protein is purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.

 Approx. Molecular Weight:84kD.

Specificity: This protein shows very high binding affinity to T.cruzi (Chagas) converted  human serum polyclonal antibodies in ELISA and  Western Elisa. Qualified for one-step immunochromatographic diagnostic tests.

Biological Activity: Not done

Application and Instructions for use

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml, 1ul/cm.