Product Specifications:

Item# 90020: Recombinant LeptospiraComposite L2 Antigen OmpL1-MLP17-LigA (E.coli) 

Concentration:  See Vial                                                  

Mass/vial: See vial                                                

Volume/vial: See Vial                                                          

Diluent: 50mM Tris, 50mM NaCl, pH9, 10%Glycerol                                             

Purity: Approx. 98%                                                                                                  

Stabilizer: None                                                                        

Preservative:None                                                                     

Storage: -75°C

Physical State: Frozen Liquid

Stability: 2 years at -75°C 

Application: In-Vitro Diagnostics, Immunization.

Description:Full length Recombinant Leptospira Composite L2 antigen- LipL32-LipL41-produced in the E.coli Expression System.

Purification:This protein is purified by solvent extraction, preparative electrophoresis and fusion tag affinity immuno-chromatography (proprietary) to >98% purity, as determined by SDS-PAGE, reduced

 Approx. Molecular Weight: 62.2kD.

Specificity: This protein binds to Leptospira converted human serum polyclonal antibodies, as determined by ELISA and Western   ELISA. Suitable for use in lateral flow human Leptospira diagnostics.

Biological Activity: Not Determined.

Application and Instructions for use

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.  Leptospira converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.