PRODUCT SPECIFICATIONS

ITEM# 7003 :  Recombinant Human APOBEC3B (E.coli)

  SPECIFICATIONS                                                                        APPLICATIONS

  Concentration: See vial                                                           Vif binding assays

   Mass/vial: 100ug                                                                    RNA binding assays

   Diluent: PBS, 0.1% Sarcosyl

   Purity: >95%     

   Stabilizer: None     

   Preservative: None

   Storage: -75oC

   Physical State: Frozen Liquid

   Stability: 12 Months at -85C

DESCRIPTION:     Produced in the E.coli Expression System as a hexa-His fusion protein.  

PURIFICATION:   This protein is purified by Ni-affinity and preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced. Approx MW: 46kD.

SPECIFICITY: This protein binds to anti-CEM15 murine monoclonal antibodies  and rabbit polyclonal antibodies as determined by Elisa and Western Elisa.

BIOLOGICAL ACTIVITY:  Binds to recombinant vif as determined by 3B-vif elisa assays using murine anti-vif mAb HRP as a secondary reagent.