PRODUCT SPECIFICATIONS
PRODUCT# 40003: Recombinant HBV Core Antigen
SPECIFICATIONS APPLICATIONS
Concentration: | 1mg/ml | Diagnostics |
Mass/vial: | 1mg | Immunization |
Volume/vial: | 100ul | T-Cell Activation |
Diluent: | 10mMNa-PO4, 20mM NaCl, pH 7 |
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Purity: | >95% |
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Stabilizer: | None |
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Preservative: | None |
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Storage: | -75C |
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Physical State: | Aqueous Solution |
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Stability: | 24 Months at -75oC |
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DESCRIPTION: Recombinant HBV core protein -183 amino acids- expressed in the E.coli Expression system.
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PURIFICATION: Proprietary. Purity > 95 % as determined by SDS_PAGE, reduced. MW: approx. 25kD |
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SPECIFICITY: Strongly binds to HBV converted human serum antibodies as determined by ELISA and Western ELISA. Protein is suitable for lateral flow diagnostic assays.
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BIOLOGICAL ACTIVITY: Not determined.
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APPLICATION AND INSTRUCTIONS FOR USE
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.
ELISA and Western ELISA require 0.1-1.0µg protein depending on the nature and affinity of the secondary detection reagent. Studies with HBV convereted human sera are performed in 1:200 to 1:10,000 antibody dilution range.
