Product Specifications:

Item# 1019: Recombinant HIV-1C gp41 Envelope Protein (E.coli)

 Concentration: See vial                                                         

 Mass/vial: 0.1mg                                                       

 Volume/vial: see vial                                                            

 Diluent: 0 mM Tris, pH8,0.002%SDS     

  Purity: >98%                                                                                                    

 Stabilizer: None                                                                        

 Preservative: None                                                                     

 Storage: -75°C

 Physical State: Frozen Liquid

 Stability:1 year at -20oC, 3 years at -75°C.  

 Application: T-Cell Activation, Immunization, In-Vitro Diagnostics.   

 Description: Truncated Recombinant HIV-1 C envelope protein gp41 produced  in the E.coli Expression System. Truncated gp41 protein mol.wt: approx. 35kD.

 Purification: This protein was purified by affinity chromatography and by preparative electrophoresis to >98% purity as determined by SDS-PAGE, reduced.

 Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western  Elisa.

 Biological Activity:  Not done

 Application and Instructions for use

 Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Sequence bold-red below expressed in E.coli.