Product Specifications:

Item# 5008: Recombinant SIVmac251 nef (Baculovirus) 

 Concentration: See vial

 Mass/vial: 100µg  

 Diluent: 50mM Tris, pH8, 150mM NaCl  

 Purity : >95% 

 Stabilizer: None

 Preservative: None

 Storage: -75°C

 Physical State: Frozen Liquid

 Stability: At least 6 months at -75°C.

 Applications: Cell Activation,Immunological Studies, Drug Screening, ELISA Western ELISA, Diagnostics

 Description: Full length Recombinant SIV mac251 nef produced as a GST fusion protein in the Baculovirus Expression System.

Purification: This recombinant protein is purified by Glutathione -affinity chromatography and the purified protein is thrombin-cleaved to produce nef of >95% purity, as determined by SDS-PAGE, reduced.

 Specificity: This protein shows reactivity with murine monoclonal antibodies and SIV converted serum polyclonal antibodies in ELISA and Western ELISA.

 Biological activity: Not done.

 Application and Instructions for use

Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 1-10ng protein depending on the nature and affinity of the test antibody.