PRODUCT SPECIFICATION

Item# 1091:  Recombinant HIV-1 MN gp41 Envelope Protein (E.coli)

SPECIFICATIONS                                                        

  Concentration:                 See vial                           s

  Mass/vial:                         100ug                                                           

  Volume/vial:                     see vial                     

  Diluent:                               10 mM Tris, pH8,0.002%SDS pH8,0.002%SDS        Purity:                                >98%                  

  Stabilizer:                          None                                                                        

  Preservative:                     None                                                                     

  Storage:                              -75oC

  Physical State:                  Frozen Liquid

  Stability:                            1 year at -20oC, 3 years at -75oC. 

  DESCRIPTION: Truncated Recombinant HIV-1 MN envelope protein gp41 produced  in the E.coli Expression System. Truncated gp41 protein mol.wt: approx. 35kD.

 APPLICATIONS:  In-Vitro Diagnostics, Immunization,  T-Cell Activation

 PURIFICATION: This protein was purified by affinity chromatography and by preparative  electrophoresis to >98% purity as determined by SDS-PAGE, reduced.

 SPECIFICITY:  This protein binds to murine monoclonal antibodies of defined epitope specificity and HIV-1 converted human serum polyclonal antibodies in ELISA and Western Elisa.

 BIOLOGICAL ACTIVITY:  Not done

APPLICATION AND INSTRUCTIONS FOR USE

Recommended concentrations for use are approximate values.  A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications.

ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent.

Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions.